What is Red Light Therapy?
A general overview of Red Light Therapy concepts and research
Written by Vladimir Heiskanen
Red light therapy (RLT) is a form of treatment in which body parts are locally irradiated with visible red light or invisible near-infrared light to treat various indications including pain, wounds and chronic diseases.
The treatment has been around for approximately 50 years, but it has become more popular during the 21st century. In the scientific community, red light therapy is nowadays most usually called photobiomodulation therapy (PBMT) or low-level laser (light) therapy (LLLT).
While previously the common knowledge in human biology has been that visible light can affect human bodily processes predominantly via the eyes, red light therapy is based on a large body of scientific findings demonstrating that light can have local effects in the irradiated tissues via a multitude of mechanisms.
More than five thousand research articles on red light therapy have been published. The tentative findings suggest that red light therapy could be potentially helpful for the ailments of brain, eyes, heart, lungs, joints, muscles, nerves and other body parts (1-7).
How does Red Light Therapy work?
The basic principle in red light therapy is that irradiating a body part with red or near-infrared can induce biological mechanisms that can locally improve cell and tissue function.
There are a plenty of articles that have aimed to summarize the mechanisms of red light therapy. The most extensive review is the Proposed Mechanisms of Photobiomodulation or Low-Level Light Therapy (2016) written by Lucas de Freitas and Michael Hamblin (8).
The primary mechanism of photobiomodulation refers to the effects that occur immediately when the cell is irradiated with light. There is still uncertainty about the main primary mechanism in red light therapy. The most common theory posits that red light is absorbed by a mitochondrial structure called cytochrome c oxidase (CCO) (9). However, this theory might not explain the effects completely, since it has been reported that even cell lines that do not express cytochrome c oxidase seem to respond to red light irradiation (10).
In addition to this popular CCO theory, there are some alternative explanations to explain intracellular photobiomodulation effects. Some evidence suggests that the effect might be related to production and release of nitric oxide (NO) from CCO or photolabile molecules such as nitrosyl hemoglobin and S-nitrosothiols (11,12). Longer wavelengths of near-infrared light (eg. 980 nm) might also affect heat-gated TRP calcium ion channels within the cells (13). It has also been tentatively suggested that red light might decrease water viscosity within mitochondrial proteins, thus improving ATP production within the cells (14).
The secondary mechanisms are the actual changes than happen as a result of red light irradiation. It has been reported that red light therapy induces numerous measurable changes in the cellular gene expression, signalling pathways, inflammatory processes and mitochondrial function (15-17).
It has been widely acknowledged that systemic chronic inflammation and mitochondrial dysfunction are likely to be major mechanisms in a most age-related chronic diseases. During the last years, there has been an increasing number of review articles suggesting the involvement of these mechanisms in the common chronic diseases such as cancer, heart disease and diabetes.
“[R]ecent research has revealed that certain social, environmental and lifestyle factors can promote systemic chronic inflammation (SCI) that can, in turn, lead to several diseases that collectively represent the leading causes of disability and mortality worldwide, such as cardiovascular disease, cancer, diabetes mellitus, chronic kidney disease, non-alcoholic fatty liver disease and autoimmune and neurodegenerative disorders.” (19)
“Mitochondrial dysfunction is a key pathophysiological component of many acute and chronic diseases.” (20)
“Mitochondrial dysfunction and oxidative stress are largely involved in aging, cancer, age-related neurodegenerative and metabolic syndrome.” (21)
The red light therapy research community appears to strongly believe that effects of red light therapy could be related to healing systemic chronic inflammation and mitochondrial dysfunction. The view is largely supported by animal research findings, in which it is common to measure markers of inflammatory and mitochondrial function.
For example, the decrease of inflammatory markers is one of the most common findings in the preclinical research on red light therapy. As summarised in one of the recent review articles: “One of the most reproducible effects of [red light therapy] is an overall reduction in inflammation, which is particularly important for disorders of the joints, traumatic injuries, lung disorders, and in the brain (...) [red light therapy] can reduce inflammation in the brain, abdominal fat, wounds, lungs, spinal cord”. (17)
There is also experimental basis to support the idea of improving mitochondrial dysfunction with red light therapy. Irradiation of cell cultures or animals with red or near-infrared light appears to beneficially affect the mitochondrial function (18). For example, studies have shown increased ATP levels, increased mitochondrial membrane potential (ΔΨm), increased cytochrome oxidase expression, increased oxygen consumption and upregulation of SIRT1/PGC1α pathway (22). It has also been shown that red light can also protect cells against mitochondrial toxins such as potassium cyanide and tetrodotoxin (23). Thus it seems plausible that red light could have positive effects on cellular energy metabolism.
The History of Red Light Therapy
It is fair to say the majority of the relevant red light therapy research has been published during the past 15 years. However, we can go more than 100 years back in history to find traces of early reports of red light therapy. For example, physicians John Harvey Kellogg and Margaret Abigail Cleaves had written their books in 1904-1910 describing the treatment of chronic fatigue, baldness and diabetes using incandescent lamps. At the same time, therapeutic practices utilizing sunlight (heliotherapy) were also relatively popular.
The history of modern red light therapy research goes back into late 1960s, when a Hungarian researcher Endre Mester showed that red laser light may increase hair growth in mice, and later published many reports of also treating human ulcers successfully (24). After Mester’s initial reports, it took several years until researchers in other countries such as USSR, Germany, USA, Italy, Japan and Israel started also reporting beneficial effects from laser light (25-30).
While the rate of publication was relatively slow up until the 90s, the pace has been steadily increasing. In the year 2000, the total number of red light therapy articles in scientific journals was approximately 500. Now in the year 2021, we have already exceeded the total number of 5,000 articles.
We can also see that by now, red light therapy research has spread to approximately 70 different countries around the world. The most important countries to publish red light therapy research have been Brazil and the USA.
United States (in 33 different States)
China, Iran, Israel, Italy, Japan, Korea, Russia, Turkey
Australia, Canada, Germany, India, Spain, Taiwan, UK
Argentina, Austria, Belgium, Czech Republic, Denmark, Egypt, France, Greece, Hungary, Netherlands, New Zealand, Norway, Poland, Romania, Saudi Arabia, Serbia, Slovakia, South Africa, Sweden, Switzerland
Bosnia-Herzegovina, Bulgaria, Chile, Colombia, Costa Rica, Croatia, Cuba, Cyprus, Finland, Indonesia, Iraq, Ireland, Jordan, Kazahkstan, Kuwait, Macedonia, Malaysia, Mexico, Monaco, Nepal, Pakistan, Portugal, Puerto Rico, Qatar, Singapore, Slovenia, Sudan, Syria, Thailand, Ukraine, United Arab Emirates, Venezuela, Yemen, Yugoslavia
In tandem with increased scientific publication rates, many books on red light therapy are also being written nowadays both for academic and non-academic audiences.
Red light therapy books for academics
Red light therapy books for laypeople
During the past few years, red light therapy has been also covered in various magazines. The news features in science-oriented magazines have usually focused on the promise of treating specific diseases with light, while the everyday magazines usually focus on supporting skin health or general well-being.
Red Light Therapy Research Overview
The available red light therapy research can be readily accessed via scientific databases such as PubMed.gov or the PBM Database Project spreadsheet.
To this date, approximately 5,000 research articles related to red light therapy have been published. The box below shortly summarizes the study types that are included in this number.
In medical research, the evaluated treatments are usually tested in laboratory animals before proceeding to clinical studies with humans. The most common laboratory research animals are rats, mice and rabbits.
Red light therapy has been evaluated for approximately 140 different ailments in animals. For most of those ailments, the study results have been relatively positive.
However, it should be acknowledged that the findings of animal studies do not always translate to benefit in humans. It has been reported that less than 8% of cancer treatments successful in animals pass the phase I trials in humans (31).
Red light therapy has been also tested in a range of human studies. The effects on more than 120 different ailments have been evaluated so far. There has been variability between the study results, some showing benefits (eg. knee osteoarthritis) and some showing no effect (eg. pain after wisdom tooth extraction) (4,32).
Note: Asterisks (*) denote the indications that have not been evaluated in randomized controlled trials (RCT)
The effect of red light therapy on some of these ailments has been tested in multiple human studies. In research literature, systematic reviews compile and describe findings from multiple studies examining the same study question that is usually “does a treatment A have an effect on a disease B”.
Numerous systematic reviews have been published on red light therapy, a major portion of them suggesting positive treatment outcomes. In some of those, the included studies have recruited more than a thousand patients in total.
Burning mouth syndrome
+ PBM decreased pain and improved quality of life
Delayed-onset muscle soreness (DOMS)
- PBM did not significantly improve delayed-onset muscle soreness
Diabetic foot ulcer
Dos Santos 2020
+ PBM decreased the ulcer size
+ PBM improved performance and recovery from exercise
+ PBM increased hair count (in men and women)
+ PBM decreased pain after the treatment and in the 2-12 week follow-up
Low back pain
- PBM did not alleviate pain or disability compared to sham
- PBM did not affect arm circumference, grip strength or pain
+ PBM alleviated pain immediately after the treatment and during the follow-up
+ PBM decreased the risk of severe oral mucositis
+/- PBM decreased periodontal probing depth only in the short-term
Dos Santos 2019
+ PBM decreased pain and improved the Foot Function Index
+/- PBM decreased overall pain but did not improve shoulder function or range of motions
Temporomandibular disorders (TMD)
+ PBM alleviated pain and improved functional outcomes of temporomandibular joint function
+ PBM alleviated pain in tennis elbow
Wisdom tooth extraction (lower jaw)
+/- PBM reduced edema but not pain or trismus
From lasers to LEDs and sunlight
One of the most important events in the history of red light therapy has been the introduction of light-emitting diodes (LEDs).
Before the year 2000, researchers had conducted their red light therapy research mainly with lasers as their light sources, and some researchers even claimed that ordinary sources of red light might not have similar effects as lasers (33).
However, an US physician Harry Whelan and his group from Wisconsin published multiple reports in the early 2000s claiming improved wound healing and other benefits from LEDs (34). Since then, a large body of evidence has been published confirming that LEDs are as suitable as lasers for red light therapy, and hundreds of reports utilizing LEDs have been already published in scientific journals (35).
It is nowadays considered likely that any light source that emits red and near-infrared light may have red light therapy-like effects. Beneficial effects have been reported even with incandescent lamps, heat lamps and halogen lamps (36-38).
Sunlight may also have health-improving effects due to the fact that a major portion of sunlight’s spectrum actually consists of red and near-infrared light. Sunlight exposure has been associated with decreased mortality and other health benefits, but it is not yet clear whether these associations are truly causal or confounded by other relevant factors (39,40).
Pursuing Systemic Health Effects
Recently there have been reports and scientific reviews suggesting that application of red light to one body part might also have favorable effects on other body parts. For example, irradiating the bodies of mice appears to protect their brain from a neurotoxin (MPTP), and irradiating tibia and iliac bones of pigs appears to protect their hearts from a myocardial infarct.
These are called “remote”, “abscocal” or “systemic” effects of red light therapy and have been reviewed in the scientific literature. The majority of these findings have been reported in animal studies (41-43).
There is some preliminary evidence that red light therapy may have positive effects on metabolic health. Pilot trials in humans have shown that exercise-related metabolic benefits such as fat loss, muscle mass maintenance and insulin sensitivity might be greater in the subjects receiving red light therapy. Results from animal studies also suggest that red light therapy may improve diet-related insulin resistance, fatty liver and adipose tissue inflammation in mice (44-47).
The idea that red light may be beneficial for metabolic health has presumably contributed to the current popularity of whole-body red light therapy with either large red LED panels. Despite the fact that majority of red light therapy research to this date has been conducted with small devices, it is likely more and more research into large panels will come in the next years.
● Red light therapy, or photobiomodulation, is an increasingly popular treatment based on irradiating the body with various light sources, most often LEDs and lasers
● Red and near-infrared light produces measurable biological effects, including anti-inflammatory signaling and improved mitochondrial function
● Five thousand studies on red light therapy have been published in scientific journals, also including high-impact journals such as The Lancet, Circulation, PNAS and Science Translational Medicine
● Evidence from systematic reviews suggest that red light therapy could be useful in the treatment of various ailments including knee pain, diabetic foot ulcers, hair loss and burning mouth syndrome. More evidence is needed to gain more confidence in these potential benefits.
● Red light has recently become popular as a means to improve general well-being, and it can be used as an additional method along with improving nutrition, sleep and other lifestyle factors.
1. Salehpour F, Mahmoudi J, Kamari F, Sadigh-Eteghad S, Rasta SH, Hamblin MR. Brain Photobiomodulation Therapy: a Narrative Review. Molecular Neurobiology [Internet]. 2018 Jan 11 [cited 2020 Nov 21];55(8):6601–36. Available from: https://pubmed.ncbi.nlm.nih.gov/29327206/
2. Photobiomodulation for the treatment of retinal diseases: a review. International Journal of Ophthalmology [Internet]. 2016 Jan 18 [cited 2020 Nov 21];9(1). Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4768515/
3. Liebert A, Krause A, Goonetilleke N, Bicknell B, Kiat H. A Role for Photobiomodulation in the Prevention of Myocardial Ischemic Reperfusion Injury: A Systematic Review and Potential Molecular Mechanisms. Scientific Reports [Internet]. 2017 Feb 9 [cited 2020 Nov 21];7(1). Available from: https://pubmed.ncbi.nlm.nih.gov/28181487/
4. Stausholm MB, Naterstad IF, Joensen J, Lopes-Martins RÁB, Sæbø H, Lund H, et al. Efficacy of low-level laser therapy on pain and disability in knee osteoarthritis: systematic review and meta-analysis of randomised placebo-controlled trials. BMJ Open [Internet]. 2019 Oct [cited 2020 Nov 21];9(10):e031142. Available from: https://pubmed.ncbi.nlm.nih.gov/31662383/
5. Nejatifard M, Asefi S, Jamali R, Hamblin MR, Fekrazad R. Probable positive effects of the photobiomodulation as an adjunctive treatment in COVID-19: A systematic review. Cytokine [Internet]. 2021 Jan [cited 2021 Jan 12];137:155312. Available from: https://pubmed.ncbi.nlm.nih.gov/33128927/
6. Leal-Junior ECP. Photobiomodulation Therapy in Skeletal Muscle: From Exercise Performance to Muscular Dystrophies. Photomedicine and Laser Surgery [Internet]. 2015 Feb [cited 2020 Nov 21];33(2):53–4. Available from: https://pubmed.ncbi.nlm.nih.gov/25654277/
7. Andreo L, Soldera CB, Ribeiro BG, de Matos PRV, Bussadori SK, Fernandes KPS, et al. Effects of photobiomodulation on experimental models of peripheral nerve injury. Lasers in Medical Science [Internet]. 2017 Oct 23 [cited 2020 Nov 21];32(9):2155–65. Available from: https://pubmed.ncbi.nlm.nih.gov/29063472/
8. de Freitas LF, Hamblin MR. Proposed Mechanisms of Photobiomodulation or Low-Level Light Therapy. IEEE Journal of Selected Topics in Quantum Electronics [Internet]. 2016 May [cited 2020 Nov 21];22(3):348–64. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5215870/
9. Karu T. Primary and secondary mechanisms of action of visible to near-IR radiation on cells. Journal of Photochemistry and Photobiology B: Biology [Internet]. 1999 Mar [cited 2020 Nov 21];49(1):1–17. Available from: https://www.sciencedirect.com/science/article/abs/pii/S101113449800219X
10. Lima PLV, Pereira CV, Nissanka N, Arguello T, Gavini G, Maranduba CM da C, et al. Photobiomodulation enhancement of cell proliferation at 660 nm does not require cytochrome c oxidase. Journal of Photochemistry and Photobiology B: Biology [Internet]. 2019 May [cited 2020 Dec 27];194:71–5. Available from: https://pubmed.ncbi.nlm.nih.gov/30927704/
11. Quirk BJ, Whelan HT. What Lies at the Heart of Photobiomodulation: Light, Cytochrome C Oxidase, and Nitric Oxide—Review of the Evidence. Photobiomodulation, Photomedicine, and Laser Surgery [Internet]. 2020 Sep 1 [cited 2020 Nov 21];38(9):527–30. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7495914/
12. Serrage H, Heiskanen V, Palin WM, Cooper PR, Milward MR, Hadis M, et al. Under the spotlight: mechanisms of photobiomodulation concentrating on blue and green light. Photochemical & Photobiological Sciences [Internet]. 2019 [cited 2020 Dec 27];18(8):1877–909. Available from: https://pubs.rsc.org/--/content/articlelanding/2019/pp/c9pp00089e/unauth#!divAbstract
13. Wang Y, Huang Y-Y, Wang Y, Lyu P, Hamblin MR. Photobiomodulation of human adipose-derived stem cells using 810 nm and 980 nm lasers operates via different mechanisms of action. Biochimica et Biophysica Acta (BBA) - General Subjects [Internet]. 2017 Feb [cited 2020 Nov 21];1861(2):441–9. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5195895/
14. Sommer AP, Haddad MKh, Fecht H-J. Light Effect on Water Viscosity: Implication for ATP Biosynthesis. Scientific Reports [Internet]. 2015 Jul 8 [cited 2020 Dec 27];5(1). Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4495567/
15. Peplow PV, Chung T-Y, Ryan B, Baxter GD. Laser Photobiomodulation of Gene Expression and Release of Growth Factors and Cytokines from Cells in Culture: A Review of Human and Animal Studies. Photomedicine and Laser Surgery [Internet]. 2011 May [cited 2020 Nov 21];29(5):285–304. Available from: https://pubmed.ncbi.nlm.nih.gov/21309703/
16. Wu S, Xing D. Intracellular signaling cascades following light irradiation. Laser & Photonics Reviews [Internet]. 2013 Apr 24 [cited 2020 Nov 21];8(1):115–30. Available from: https://onlinelibrary.wiley.com/doi/abs/10.1002/lpor.201300015
17. R Hamblin M. Mechanisms and applications of the anti-inflammatory effects of photobiomodulation. AIMS Biophysics [Internet]. 2017 [cited 2020 Nov 21];4(3):337–61. Available from: http://www.aimspress.com/article/10.3934/biophy.2017.3.337
18. Hamblin MR. Mechanisms and Mitochondrial Redox Signaling in Photobiomodulation. Photochemistry and Photobiology [Internet]. 2018 Jan 19 [cited 2020 Nov 21];94(2):199–212. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5844808/
19. Furman D, Campisi J, Verdin E, Carrera-Bastos P, Targ S, Franceschi C, et al. Chronic inflammation in the etiology of disease across the life span. Nature Medicine [Internet]. 2019 Dec [cited 2020 Nov 21];25(12):1822–32. Available from: https://www.nature.com/articles/s41591-019-0675-0?fbclid=IwAR3DAUfM0Ee0gnHOGBU0juIEfsvkDAXQ3Ew1RY0ORRWmjZtkXCQzPW-wZkg
20. Mitochondrial Biogenesis as a Pharmacological Target: A New Approach to Acute and Chronic Diseases [Internet]. Annual Reviews. 2019 [cited 2020 Nov 21]. Available from: https://www.annualreviews.org/doi/abs/10.1146/annurev-pharmtox-010715-103155
21. Bhatti JS, Bhatti GK, Reddy PH. Mitochondrial dysfunction and oxidative stress in metabolic disorders — A step towards mitochondria based therapeutic strategies. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease [Internet]. 2017 May [cited 2020 Nov 21];1863(5):1066–77. Available from: https://pubmed.ncbi.nlm.nih.gov/27836629/
22. Zhang Z, Shen Q, Wu X, Zhang D, Xing D. Activation of PKA/SIRT1 signaling pathway by photobiomodulation therapy reduces Aβ levels in Alzheimer’s disease models. Aging Cell [Internet]. 2019 Oct 30 [cited 2020 Nov 21];19(1). Available from: https://pubmed.ncbi.nlm.nih.gov/31663252/
23. Wong-Riley MTT, Liang HL, Eells JT, Chance B, Henry MM, Buchmann E, et al. Photobiomodulation Directly Benefits Primary Neurons Functionally Inactivated by Toxins. Journal of Biological Chemistry [Internet]. 2004 Nov 22 [cited 2020 Nov 21];280(6):4761–71. Available from: https://pubmed.ncbi.nlm.nih.gov/15557336/
24. The History of Photobiomodulation: Endre Mester (1903–1984) [Internet]. Photomedicine and Laser Surgery. 2013 [cited 2020 Nov 21]. Available from: https://www.liebertpub.com/doi/abs/10.1089/pho.2017.4332
25. Gamaleya NF. Laser Biomedical Research in the USSR. Laser Applications in Medicine and Biology [Internet]. 1977 [cited 2020 Nov 21];1–173. Available from: https://link.springer.com/chapter/10.1007/978-1-4615-7326-5_1
26. Kana JS. Effect of Low—Power Density Laser Radiation on Healing of Open Skin Wounds in Rats. Archives of Surgery [Internet]. 1981 Mar 1 [cited 2020 Nov 21];116(3):293. Available from: https://pubmed.ncbi.nlm.nih.gov/7469766/
27. Goldman JA, Chiapella J, Bass N, Graham J, McClatchey W, Dronavalli RV, et al. Laser therapy of rheumatoid arthritis. Lasers in Surgery and Medicine [Internet]. 1980 [cited 2020 Nov 21];1(1):93–101. Available from: https://pubmed.ncbi.nlm.nih.gov/7038361/
28. Bosatra M, Jucci A, Olliaro P, Quacci D, Sacchi S. In vitro Fibroblast and Dermis Fibroblast Activation by Laser Irradiation at Low Energy. Dermatology [Internet]. 1984 [cited 2020 Nov 21];168(4):157–62. Available from: https://pubmed.ncbi.nlm.nih.gov/6724069/
29. Kami T, Yoshimura Y, Nakajima T, Ohshiro T, Fujino T. Effects of Low-Power Diode Lasers on Flap Survival. Annals of Plastic Surgery [Internet]. 1985 Mar [cited 2020 Nov 21];14(3):278–83. Available from: https://pubmed.ncbi.nlm.nih.gov/3994272/
30. Rochkind S, Nissan M, Razon N, Schwartz M, Bartal A. Electrophysiological effect of HeNe laser on normal and injured sciatic nerve in the rat. Acta Neurochirurgica [Internet]. 1986 Sep [cited 2020 Nov 21];83(3–4):125–30. Available from: https://pubmed.ncbi.nlm.nih.gov/3028047/
31. Mak IW, Evaniew N, Ghert M. Lost in translation: animal models and clinical trials in cancer treatment. American journal of translational research [Internet]. 2014 [cited 2020 Nov 21];6(2):114–8. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3902221/
32. Domah F, Shah R, Nurmatov UB, Tagiyeva N. The Use of Low-Level Laser Therapy to Reduce Postoperative Morbidity After Third Molar Surgery: A Systematic Review and Meta-Analysis. Journal of Oral and Maxillofacial Surgery [Internet]. 2020 Sep [cited 2020 Nov 21]; Available from: https://pubmed.ncbi.nlm.nih.gov/33058775/
33. Hode L. The Importance of the Coherency. Photomedicine and Laser Surgery [Internet]. 2005 Aug [cited 2020 Nov 21];23(4):431–4. Available from: https://pubmed.ncbi.nlm.nih.gov/16144489/
34. Whelan H;Desmet K;Buchmann E;Henry M;Wong-Riley M;Eells J;Verhoeve J. Harnessing the cell’s own ability to repair and prevent neurodegenerative disease. SPIE newsroom [Internet]. 2016 [cited 2020 Nov 21];2008. Available from: https://pubmed.ncbi.nlm.nih.gov/19265872/
35. Heiskanen V, Hamblin MR. Photobiomodulation: lasers vs. light emitting diodes? Photochemical & Photobiological Sciences [Internet]. 2018 [cited 2020 Dec 27];17(8):1003–17. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6091542/
36. Park D, Kyung J, Kim D, Hwang S-Y, Choi E-K, Kim Y-B. Anti-hypercholesterolemic and anti-atherosclerotic effects of polarized-light therapy in rabbits fed a high-cholesterol diet. Laboratory Animal Research [Internet]. 2012 [cited 2020 Nov 21];28(1):39. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3315201/
37. Petrofsky JS, Lawson D, Berk L, Suh H. Enhanced healing of diabetic foot ulcers using local heat and electrical stimulation for 30 min three times per week. Journal of Diabetes [Internet]. 2010 Mar [cited 2020 Nov 21];2(1):41–6. Available from: https://onlinelibrary.wiley.com/doi/full/10.1111/j.1753-0407.2009.00058.x
38. Verbelen J. Use of polarised light as a method of pressure ulcer prevention in an adult intensive care unit. Journal of Wound Care [Internet]. 2007 Apr [cited 2020 Nov 21];16(4):145–50. Available from: https://pubmed.ncbi.nlm.nih.gov/17444378/
39. Heiskanen V, Pfiffner M, Partonen T. Sunlight and health: shifting the focus from vitamin D3 to photobiomodulation by red and near-infrared light. Ageing Research Reviews [Internet]. 2020 Aug [cited 2020 Nov 21];61:101089. Available from: https://pubmed.ncbi.nlm.nih.gov/32464190/
40. Lindqvist PG, Epstein E, Landin-Olsson M, Ingvar C, Nielsen K, Stenbeck M, et al. Avoidance of sun exposure is a risk factor for all-cause mortality: results from the Melanoma in Southern Sweden cohort. Journal of Internal Medicine [Internet]. 2014 Apr 23 [cited 2020 Nov 21];276(1):77–86. Available from: https://pubmed.ncbi.nlm.nih.gov/24697969/
41. Johnstone DM, el Massri N, Moro C, Spana S, Wang XS, Torres N, et al. Indirect application of near infrared light induces neuroprotection in a mouse model of parkinsonism – An abscopal neuroprotective effect. Neuroscience [Internet]. 2014 Aug [cited 2020 Nov 21];274:93–101. Available from: https://pubmed.ncbi.nlm.nih.gov/24857852/
42. Blatt A, Elbaz-Greener GA, Tuby H, Maltz L, Siman-Tov Y, Ben-Aharon G, et al. Low-Level Laser Therapy to the Bone Marrow Reduces Scarring and Improves Heart Function Post-Acute Myocardial Infarction in the Pig. Photomedicine and Laser Surgery [Internet]. 2016 Nov [cited 2020 Nov 21];34(11):516–24. Available from: https://pubmed.ncbi.nlm.nih.gov/26741110/
43. Kim B, Brandli A, Mitrofanis J, Stone J, Purushothuman S, Johnstone DM. Remote tissue conditioning — An emerging approach for inducing body-wide protection against diseases of ageing. Ageing Research Reviews [Internet]. 2017 Aug [cited 2020 Nov 21];37:69–78. Available from: https://www.sciencedirect.com/science/article/pii/S1568163717300053
44. Sene-Fiorese M, Duarte FO, de Aquino Junior AE, Campos RM da S, Masquio DCL, Tock L, et al. The potential of phototherapy to reduce body fat, insulin resistance and “metabolic inflexibility” related to obesity in women undergoing weight loss treatment. Lasers in Surgery and Medicine [Internet]. 2015 Jul 29 [cited 2020 Nov 21];47(8):634–42. Available from: https://pubmed.ncbi.nlm.nih.gov/26220050/
45. Silva G, Ferraresi C, Almeida RT, Motta ML, Paixão T, Ottone VO, et al. Insulin resistance is improved in high‐fat fed mice by photobiomodulation therapy at 630 nm. Journal of Biophotonics [Internet]. 2020 Jan 7 [cited 2020 Nov 21];13(3). Available from: https://pubmed.ncbi.nlm.nih.gov/31707768/
46. Guo S, Gong L, Shen Q, Xing D. Photobiomodulation reduces hepatic lipogenesis and enhances insulin sensitivity through activation of CaMKKβ/AMPK signaling pathway. Journal of Photochemistry and Photobiology B: Biology [Internet]. 2020 Dec [cited 2020 Nov 21];213:112075. Available from: https://pubmed.ncbi.nlm.nih.gov/33152638/
47. Yoshimura TM, Sabino CP, Ribeiro MS. Photobiomodulation reduces abdominal adipose tissue inflammatory infiltrate of diet-induced obese and hyperglycemic mice. Journal of Biophotonics [Internet]. 2016 Sep 16 [cited 2020 Nov 21];9(11–12):1255–62. Available from: https://pubmed.ncbi.nlm.nih.gov/27635634/